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Im the one that was paying for Netflix for my family, but the password crack down motivated me to learn how to build a server and go full arrs. They had a good thing going, but now that $26 a month will be used to buy hdds.
Im the one that was paying for Netflix for my family, but the password crack down motivated me to learn how to build a server and go full arrs. They had a good thing going, but now that $26 a month will be used to buy hdds.
Lol yeh but it’s partially funded by money saved from canceled subscriptions.
I’m a noob, I started 2 months ago with immich and tailscale. Now I have an unraid server, it’s a slippery slope.
Pretty good description. Few extra points. -you don’t need to don’t need to do PCR to have enough Nucleic acids to visualize on the gel. Often you use gels to visualize things like purified plasmids. You can also digest your plasmids with enzymes to confirm genomic insert. -these type of gels have many uses outside of direct comparisons, for example you can use the gel purity DNA of a particular size by cutting out the band, melting the gel and using a silica column to pull the DNA out of the melted gel slice. This is super common when you do molecular cloning.
For what is possibly wrong with the top gel.
-the blurred bands can be a lot of things. It might just be inherited to the sample type, for example raw genomic DNA will look like this because you’ll have enzyme that degrade DNA, and a lot of mid replication chromosomes resulting in fragments existing across a spectrum of sizes equally. Additionally the it could also be user error with overloaded well or not enough loading buffer in the sample so it’s not pushed in to gel evenly, or it was run to fast.
Overall the top gel just looks like crap and the bottom gel looks great, but depending on what was run you really don’t know what the gel should look like, if I was testing endonuclease activity the top gel would probably be what I was hoping to get. If was doing PCR on single gene, the bottom one is better.
Most definitely and probably be missing some limb by now.
Star wars has been effectively marketing me lightsabers for years, and I can’t even carve a turkey with mine.
I paid $1000 for books my first semester of college back in 2007. I felt so burnt and violated I never bought another textbook. I made it through the rest of undergrad, a masters, and a PhD in biochemistry by checking out books from the library, borrowing textbooks from friends, and going sailing. When I taught I made it a point to teach my students about all the ways they can avoid becoming a victim like myself.
The everything is woke or not is out of control, when the real wide spread problem is just bad writing and story telling. I’m just tired of stories with contrived conflicts that occur just to make the story move forward “ie the entire plot of God of war Ragnarok”, and established characters making uncharacteristic choices and actions without any development towards that change, “ie Luke Skywalker in the last Jedi and basically everyone in the last season of game of thrones”.
Buying my own Costco membership was the first moment I truly felt like an adult.
That’s probably more of a reason to get her vaccinated. Those topical flea and tick meds are rough. We got our cat vaccinated even though she was 100% indoor because she used to get too sick with topicals and liked to cuddle the dog.
Poor thing, I’m glad he has a loving family now. I once found a golden retriever wandering the forest while hiking in Indiana. She had so many ticks on her we had to take her to an emergency vet (since it was Sunday) to get them off. Luckly she was microchipped and we found her family who has been looking for her for over a week at the time.
If only there was a vaccine for Lyme disease so the poor pupper wouldn’t have had to suffer in the first place
I’ve repaired about 20 different Gameboys. The community for Gameboy repair and mods is huge. I suggest watching a few of macho nacho videos on YouTube to work up the nerves to open it. They are so easy to fix, and there is a modern replacement for almost anything you can break. Also take a look at the ifixit guides. https://www.ifixit.com/Device/Game_Boy
Can we do radio shack from the 80s early 90s over the cellphone retailer they turned into before death.
Prehensile penis is the unachievable body standard for the dense folk out there
I’m sorry Jon
You have to add kindling to build the fire, and thats why I try not to only lurk here.
That’s clearly a gate to the clouds, not a bean. 🙃
Spez the hero of Lemmy
My alignment is neutral good